|Product Overview:||Recombinant Human TGFB1, Ala279-Ser390, was produced in CHO cell under cGMP guidelines.|
|Form:||Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.|
|Bio-activity:||Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The specific activity of recombinant human TGF-beta 1 GMP is approximately 2.5 x 10^4 U/μg, which is calibrated against human TGF‑ beta 1 Standard (NIBSC code: 89/514).|
|Molecular Mass:||Predicted Molecular Mass: 12.8 (monomer) kDa
SDS-PAGE: 12 kDa, reducing conditions; 24 kDa, non-reducing conditions
|N-terminal Sequence Analysis:||Ala-Leu-Asp-Thr-Asn-Tyr-(Cys)-Phe-Ser-Ser|
|Endotoxin:||<0.10 EU per 1 μg of the protein by the LAL method.|
|Purity:||>97%, by SDS-PAGE with silver staining.|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 100 μg/mL in 4 mM HCl.|
|Quality Statement:||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping:||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name:||TGFB1 transforming growth factor, beta 1 [ Homo sapiens ]|
|Synonyms:||TGFB1; transforming growth factor, beta 1; DPD1, TGFB; transforming growth factor beta-1; Camurati Engelmann disease; CED; TGFbeta; TGF-beta-1; TGF-beta 1 protein; LAP; DPD1; TGFB;|