Active GMP Recombinant Human TNFSF10 protein, Fc-tagged

• Cat.No.: TNFSF10-022HG
• Product Overview: GMP Recombinant Human TNFSF10 protein(NP_003801.1)(405 aa), fused with Fc Tag at the C-terminal, was expressed in HEK293 cell in an animal component free process under cGMP guidelines.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Bio-activity: Determined by the ability to induce apoptosis in TRAIL-sensitive U343MG cells, the expected ED50 of this effect is 1.0~3.0ng/ml. Taking its ability to induce apoptosis of LN-18 cells (human glioblastoma cells) as an indicator, the expected ED50 of this effect is 0.8~2.0ng/ml.
• Molecular Mass: Human TNFSF10 contains 405 aa. It has a predicted MW of 46.3 kDa.
• Protein length: DNA sequence encoding Human sTRAIL(NP_003801.1) was expressed with Fc tag at the C-terminal.
• Endotoxin: <=0.01EU/ug
• Purity: >=95% by SDS-PAGE and HPLC
• Storage: The lyophilized preparation can be stored at 4°C for 24 months, and the dissolved liquid can be stored at -20°C for 6-12 months to avoid repeated freezing and thawing
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TNFSF10 tumor necrosis factor (ligand) superfamily, member 10 [ Homo sapiens ]
• Official Symbol: TNFSF10
• Synonyms: CED; DPD1; LAP; TGF-beta1; TGFB; TGFbeta
• Gene ID: 8743
• mRNA Refseq: NM_001190942
• Protein Refseq: NP_001177871
• MIM: 603598
• UniProt ID: P50591