|Product Overview:||Recombinant Human WNT3A, Ser19-Lys352, was produced in CHO cell under cGMP guidelines.|
|Form:||Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.|
|Bio-activity:||Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells.|
|Molecular Mass:||Predicted Molecular Mass: 37.4 kDa
SDS-PAGE: 40 kDa reducing conditions
|N-terminal Sequence Analysis:||Ser19-Tyr-Pro-Ile-Trp-Trp-Ser-Leu-Ala-Val|
|Endotoxin:||<0.10 EU per 1 μg of the protein by the LAL method.|
|Purity:||>75%, by SDS-PAGE with silver staining, under reducing conditions.|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 200 μg/mL in PBS.|
|Quality Statement:||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping:||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name:||WNT3A wingless-type MMTV integration site family, member 3A [ Homo sapiens ]|
|Synonyms:||WNT3A; wingless-type MMTV integration site family, member 3A; protein Wnt-3a; MGC119418; MGC119419; MGC119420;|