Active GMP Recombinant Human WNT3A Protein

• Cat.No.: WNT3A-131HG
• Product Overview: Recombinant Human WNT3A, Ser19-Lys352, was produced in CHO cell under cGMP guidelines.

Specification

• Source: CHO cell
• Species: Human
• Form: Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
• Bio-activity: Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells.
• Molecular Mass: Predicted Molecular Mass: 37.4 kDa
  SDS-PAGE: 40 kDa reducing conditions
• N-terminal Sequence Analysis: Ser19-Tyr-Pro-Ile-Trp-Trp-Ser-Leu-Ala-Val
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >75%, by SDS-PAGE with silver staining, under reducing conditions.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 200 μg/mL in PBS.
• Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Gene Information

• Gene Name: WNT3A wingless-type MMTV integration site family, member 3A [ Homo sapiens ]
• Official Symbol: WNT3A
• Synonyms: WNT3A; wingless-type MMTV integration site family, member 3A; protein Wnt-3a; MGC119418; MGC119419; MGC119420
• Gene ID: 89780
• mRNA Refseq: NM_033131
• Protein Refseq: NP_149122
• MIM: 606359
• UniProt ID: P56704