Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Active GMP Recombinant Porcine IL1B Protein, His-Tagged

Cat.No. : IL1B-01P
Product Overview : GMP Recombinant Porcine IL1B Protein, His-Tagged was expressed in Escherichia coli cell-derived in an animal component free process under cGMP guidelines.
  • Specification
  • Gene Information
  • Related Products
Description : Interleukin 1 beta (IL-1β) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a cytokine protein that in humans is encoded by the IL1B gene. There are two genes for interleukin-1 (IL-1): IL-1 alpha and IL-1 beta (this gene). IL-1β precursor is cleaved by cytosolic caspase 1 (interleukin 1 beta convertase) to form mature IL-1β.
Source : Escherichia coli
Species : Porcine
Tag : His
Form : Lyophilized
Bio-activity : Measure by its ability to induce D10.G4.1 cells proliferation. The ED50 for this effect is <3 ng/mL.
AA Sequence : MANVQSMECKLQDKDHKSLVLAGPH MLKALHLLTGDLKREVVFCMSFVQG DDSNNKIPVTLGIKGKNLYLSCVMK DNTPTLQLEDIDPKRYPKRDMEKRF VFYKTEIKNRVEFESALYPNWYIST SQAEQKPVFLGNSKGRQDITDFTME VLSP with polyhistidine tag at the C-terminus
Endotoxin : <0.1 EU per 1 μg of the protein by the LAL method.
Purity : >98% as determined by SDS-PAGE. Ni-NTA chromatography
Notes : Please use within one month after protein reconstitution.
Storage : Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
Storage Buffer : The protein was lyophilized from a solution containing 1X PBS, pH 7.4.
Reconstitution : It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Gene Name : IL1B interleukin 1 beta [ Sus scrofa (pig) ]
Official Symbol : IL1B
Synonyms : IL1B1
Gene ID : 397122
mRNA Refseq : NM_214055.1
Protein Refseq : NP_999220.1
UniProt ID : P26889

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (10)

Ask a question
How does IL-1β initiate inflammasome assembly, and which experimental techniques unravel this intricate process? 07/30/2022

Inflammasome assembly initiated by IL-1β is deciphered using techniques like co-immunoprecipitation, proximity ligation assays, and super-resolution microscopy.

What are the specific mechanisms underlying IL-1β's priming of immune cells, and how can flow cytometry and gene expression analyses decipher these molecular pathways? 07/08/2021

IL-1β's priming of immune cells is dissected through flow cytometry, RNA sequencing, and chromatin immunoprecipitation, revealing its epigenetic and transcriptional effects.

How do post-translational modifications regulate IL-1β's biological activity, and how can mass spectrometry-based phosphoproteomics provide insights into these modifications? 06/06/2020

Mass spectrometry-based phosphoproteomics deciphers IL-1β's post-translational modifications, revealing regulatory sites crucial for its activation and signaling.

How can advanced bioinformatics tools be applied to multi-omics data to comprehensively understand the intricate roles of IL-1β in health and disease? 04/16/2020

Bioinformatics tools integrate multi-omics data to provide a comprehensive understanding of IL-1β's roles, revealing its intricate contributions to health and disease.

What is the significance of IL-1β's epigenetic regulation in chronic inflammatory diseases, and how can ChIP-seq and DNA methylation profiling uncover these regulatory elements? 11/01/2019

ChIP-seq and DNA methylation profiling uncover IL-1β's epigenetic impact on target genes, shedding light on its role in shaping chronic inflammatory states.

How does IL-1β interact with its downstream effectors to induce the production of pro-inflammatory cytokines, and how can dual-luciferase reporter assays reveal these intricate interactions? 03/31/2019

IL-1β's interactions with downstream effectors are unveiled using dual-luciferase reporter assays, surface plasmon resonance, and co-crystallization studies.

What are the complex feedback loops between IL-1β and other cytokines, and how can systems biology approaches like network analysis dissect these dynamic interactions? 01/24/2019

Network analysis integrates omics data to unravel IL-1β's intricate cross-talk with other cytokines, mapping out complex regulatory loops in inflammation.

What is the role of IL-1β in modulating the tumor microenvironment, and how can advanced imaging techniques like intravital microscopy elucidate its impact on immune cell dynamics? 08/08/2017

IL-1β's impact on the tumor microenvironment is studied using intravital microscopy, 3D culture models, and single-cell RNA sequencing to capture dynamic cell behavior.

What are the mechanisms underlying IL-1β's non-canonical signaling pathways, and how can CRISPR-Cas9 screening identify key mediators of these alternative pathways? 04/18/2017

CRISPR-Cas9 screening identifies IL-1β's non-canonical signaling mediators through functional genomics, revealing alternative pathways in cellular responses.

How does IL-1β's signaling adapt to different cellular contexts, and how can single-cell RNA sequencing uncover cell-specific responses to this cytokine? 01/03/2017

Single-cell RNA sequencing unveils IL-1β's context-specific responses, exposing cell type-specific signaling and gene expression patterns.

Customer Reviews (3)

Write a review
Reviews
11/08/2021

    Effective in inflammation studies.

    04/23/2021

      High specificity.

      06/30/2020

        Suitable for cytokine assays.

        Ask a Question for All IL1B Products

        Required fields are marked with *

        My Review for All IL1B Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends