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Active GMP Recombinant Porcine IL4 Protein, His-Tagged

Cat.No. : IL4-01P
Product Overview : GMP Recombinant Porcine IL4 Protein, His-Tagged was expressed in Escherichia coli cell-derived in an animal component free process under cGMP guidelines.
  • Specification
  • Gene Information
  • Related Products
Description : The interleukin 4 (IL4, IL-4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4 in a positive feedback loop. The cell that initially produces IL-4, thus inducing Th2 differentiation, has not been identified, but recent studies suggest that basophils may be the effector cell. It is closely related and has functions similar to Interleukin 13.
Source : Escherichia coli
Species : Porcine
Tag : His
Form : Lyophilized
Bio-activity : Measure by its ability to induce proliferation in TF-1 cells. The ED50 for this effect is <0.6 ng/mL.
AA Sequence : MHKCDITLQEIIKTLNILTARKNSC MELPVTDVFAAPENTTEKETFCRAS TVLRHIYRHHTCMKSLLSGLDRNLS SMANMTCSVHEAKKSTLKDFLERLK TIMKEKYSKC with polyhistidine tag at the C-terminus
Endotoxin : <0.1 EU per 1 μg of the protein by the LAL method.
Purity : >98% as determined by SDS-PAGE. Ni-NTA chromatography
Notes : Please use within one month after protein reconstitution.
Storage : Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
Storage Buffer : The protein was lyophilized from a solution containing 1X PBS, pH 7.4.
Reconstitution : It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Gene Name : IL4 interleukin 4 [ Sus scrofa (pig) ]
Official Symbol : IL4
Gene ID : 397225
mRNA Refseq : NM_214123.1
Protein Refseq : NP_999288.1
UniProt ID : Q04745

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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10/17/2021

    By testing immunogenicity in animal models, detect whether an immune response is induced.

    11/09/2020

      The structure of the recombinant protein was analyzed by nuclear magnetic resonance (NMR), X-ray crystallography or electron microscopy, and the results showed that the protein was stable.

      05/06/2020

        This protein has good stability, and the test results of heat resistance, acid and alkali resistance and protease degradation resistance are good.

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