| Description : |
Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. In humans, it is encoded by the IL6 gene. In addition, osteoblasts secrete IL-6 to stimulate osteoclast formation. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6 as a pro-inflammatory cytokine. IL-6's role as an anti-inflammatory myokine is mediated through its inhibitory effects on TNF-alpha and IL-1, and activation of IL-1ra and IL-10. |
| Source : |
Escherichia coli |
| Species : |
Porcine |
| Tag : |
His |
| Form : |
Lyophilized |
| Bio-activity : |
Measure by its ability to induce proliferation in T1165.85.2.1 cells. The ED50 for this effect is <1.5 ng/mL. |
| AA Sequence : |
MGRLEEDAKGDATSDKMLFTSPDKT EELIKYILGKISAMRKEMCEKYEKC ENSKEVLAENNLNLPKMAEKDGCFQ SGFNQETCLMRITTGLVEFQIYLDY LQKEYESNKGNVEAVQISTKALIQT LRQKGKNPDKATTPNPTTNAGLLDK LQSQNEWMKNTKIILILRSLEDFLQ FSLRAIRIM with polyhistidine tag at the C-terminus |
| Endotoxin : |
<0.1 EU per 1 μg of the protein by the LAL method. |
| Purity : |
>98% as determined by SDS-PAGE. Ni-NTA chromatography |
| Notes : |
Please use within one month after protein reconstitution. |
| Storage : |
Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C. |
| Storage Buffer : |
The protein was lyophilized from a solution containing 1X PBS, pH 7.4. |
| Reconstitution : |
It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved. |
