Active Human IL8

• Cat.No.: IL8-104H
• Product Overview: It is a synthetic Protein

Specification

• Description: Interleukin 8 (IL-8), formerly called monocyte-derived neutrophil chemotactic factor, belongs to the C-X-C chemokine family. It was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil activating protein, neutrophil chemotactic factor, leukocyte adhesion inhibitor, granulocyte chemotactic protein and T-lymphocyte chemotactic factor. IL-8 consists of 72 amino acids with a molecular weight of 8 kDa. IL-8 exhibits chemotactic activity in vitro for T cells, basophils and neutrophils. IL-8 activates neutrophils to release lysosomal enzymes including myeloperoxidase, -mannosidase and -glucuronidase. IL-1, TNF, Lipopolysaccharides and viruses induce the production of IL-8 from fibroblasts, keratinocytes, endothelial cells, hepatoma cells, astrocytoma cells, glioblastoma cells, lung epithelial cells, synovial membrane cells, melanocytes, melanoma cells and gastric cancer cells.
• Species: Human
• Form: Lyophilized
• Bio-activity: Activity measured by ability of the protein to induce myeloperoxidase release from human neutrophils or chemotaxis of hCXCR-2 transfected mouse BaF/3 cells. ED50s for these measurements are generally 0.2-0.4 μg/ml and 0.2-1 ng/ml, respectively.
• Applications: Activity Assay
• Storage: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
• Reconstitution: Recommended reconstitution in sterile phosphate buffered saline which contains at least 0.1% human or bovine serum albumin to prepare a stocksolution of no less than 20 μg/ml and vortex thoroughly.
• Publication:
  Silicon Nanowire Biosensor for Highly Sensitive and Multiplexed Detection of Oral Squamous Cell Carcinoma Biomarkers in Saliva. (2015)
  Endogenous IL-8 acts as a CD16 co-activator for natural killer-mediated anti-CD20 B cell depletion in chronic lymphocytic leukemia (2013)
  Oscilatory PEEP (BiPEEP) Improves Oxygenation and Reduces Inflammation in an Experimental Model of ARDS (2023)

Gene Information

• Gene Name: IL8 interleukin 8 [ Homo sapiens ]
• Official Symbol: IL8
• Synonyms: IL8; interleukin 8; interleukin-8; 3 10C; alveolar macrophage chemotactic factor I; AMCF I; b ENAP; beta endothelial cell derived neutrophil activating peptide; chemokine (C X C motif) ligand 8; CXCL8; GCP 1; GCP1; granulocyte chemotactic protein 1; IL 8; K60; LECT; LUCT; lung giant cell carcinoma derived chemotactic protein; lymphocyte derived neutrophil activating peptide; LYNAP; MDNCF; MONAP; monocyte derived neutrophil chemotactic factor; monocyte derived neutrophil activating peptide; NAF; NAP 1; NAP1; neutrophil activating peptide 1; SCYB8; TSG 1; tumor necrosis factor induced gene 1; emoctakin; T-cell chemotactic factor; neutrophil-activating peptide 1; chemokine (C-X-C motif) ligand 8; beta-thromboglobulin-like protein; tumor necrosis factor-induced gene 1; monocyte-derived neutrophil chemotactic factor; monocyte-derived neutrophil-activating peptide; small inducible cytokine subfamily B, member 8; lung giant cell carcinoma-derived chemotactic protein; beta endothelial cell-derived neutrophil activating peptide; GCP-1; NAP-1
• Gene ID: 3576
• mRNA Refseq: NM_000584
• Protein Refseq: NP_000575
• MIM: 146930
• UniProt ID: P10145
• Chromosome Location: 4q13-q21
• Pathway: ATF-2 transcription factor network, organism-specific biosystem; Activation of Genes by ATF4, organism-specific biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem
• Function: chemokine activity; interleukin-8 receptor binding; protein binding