JARID Demethylase Activity/Inhibition Colorimetric Assay Kit

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JARID Demethylase Activity/Inhibition Colorimetric Assay Kit

JARID Demethylase Related Products

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Cat.No. : Kit-0485
Product Overview : JARID Demethylase Activity/Inhibition Assay Kit (Colorimetric) is use for measuring activity or inhibition of total JARID.
Applications : JARID Demethylase Activity/Inhibition Assay Kit (Colorimetric) is suitable for measuring activity or inhibition of total JARID usingnuclear extracts or subtype JARID (JARID1A-JARID1D) purified enzymes from a broad range of species such as mammalians, plant, fungal, and bacterial types, in a variety of forms including cultured cells and fresh tissues. Nuclear extracts can be prepared by using your own successful method. Nuclear extracts can be used immediately or stored at –80°C for future use. Purified enzymes can be active JARIDs from recombinant proteins or isolated from cell/tissues.
Storage : Upon receipt: (1) Store JC3, JC4, and JC6 at –20°C away from light; (2) Store JC1, JC5, JC7, Co-Factor 1, Co-Factor 2, Co-Factor 3, and 8-Well Assay Strips at 4°C away from light; (3) Store remaining components (JC2, JC8, and Adhesive Covering Film) at room temperature away from light.All components of the kit are stable for 6 months from the date of shipment, when stored properly.Note: (1) Check if JC1 (10X Wash Buffer) contains salt precipitates before use. If so, warm (at room temperature or 37°C) and shake the buffer until the salts are re-dissolved; and (2) check if a blue color is present in JC7 (Developer Solution), which indicates contamination of the solution and should not be used. To avoid contamination, transfer the amount of JC7 required into a secondary container (tube or vial) before adding JC7 into the assay wells.

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