"Pertussis toxin" Related Products

Native B. pertussis Pertussis toxin

Cat.No.: PTX-107B
Product Overview: Pertussis toxin is a protein isolated from the Gramnegative coccobaccillus, Bordatella pertussis. The toxin is released in an inactive form. This product consists of 5 subunits.
Description: Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt. This prevents the G protein heterotrimers from interacting with receptors, thus blocking their coupling and activation. Since the Gα subunits remain in their GDP-bound, inactive state, they are unable to inactivate adenylyl cyclase or open K+ channels.
Source: B. pertussis
Species: B. pertussis
Form: Lyophilized powder containing sodium chloride and sodium phosphate buffer salts.
Molecular Mass: Molecular mass: ~117 kDa (ultracentrifugation)
Pertussis toxin consists of 5 subunits combined in a 1:1:1:2:1 ratio, with the following molecular masses: S1 = 26,017 Da; S2 = 21,839 Da; S3 = 21,751 Da; S4 = 12,061 Da; S5 = 11,747 Da.
Applications: The toxin is released from B. pertussis in an inactive form. When the pertussis toxin B oligomer binds to the cell membrane, the S1 subunit of its A protomer becomes activated, perhaps through the action of glutathione and ATP. A protocol for activating pertussis toxin in vitro is given by Kaslow, et al.
Storage: Store at 2-8 centigrade
Reconstitution: When reconstituted with 500 ml of water each vial will contain 0.05 M sodium phosphate, pH 7.2, with 0.5 M sodium chloride.
Handling: This pertussis toxin product has not been preactivated with ATP. Preincubate pertussis toxin in the presence of 1-5 mM ATP and 1–5 mM DTT for in vitro use with cell membranes. Preincubation with ATP and DTT is not recommended for use with intact cells or in vivo use.
Synonyms: Islet Activating Protein; Pertussigen; Leukocytosis-Promoting Factor; Lymphocytosis Promoting Factor; Histamine-Sensitizing Factor; Pertussis Exotoxin; PTX; PT
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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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