|Cat.No.:||Shiga Toxin 1-208|
|Product Overview:||Shiga Toxin 1 was purified from Escherichia coli.|
|Description:||Shiga toxin is a group of related toxins produced by Shigella dysenteriae and enterohemorrhagic Escherichia coli (EHEC). In the past, shiga toxins have been differentiated as Shiga, Shiga-like or vero toxins but since the toxins produced by the two types of bacteria are very similar, distinctions are no longer made.
Shiga toxin is produced by specific strains of Escherichia coli in two isoforms, Shiga 1(Stx1) and Shiga 2(Stx2) which share ~60% sequence homology. These Shiga toxins have the same molecular structures, enzymatic activities, receptor specificity and intracellular trafficking process. Shiga toxins are composed of two subunits, an enzymatically active A subunit which is non-covalently attached to the pentameric B subunit, responsible for binding to the target cells. The sugar domain of the glycosphingolipid, globotriaosylceramide, Gb3, located on cell surfaces, is specifically bound by the B-subunit. The shiga toxin is internalized by endocytosis and is transported via a retrograde pathway in the Golgi apparatus to the endoplasmic reticulum. The enzymatic A subunit is then translocated to the cytosol where its N-glycosidase activity irreversibly modifies ribosomal 28S RNA leading to inhibition of protein synthesis and causing cell death. The potential use of Shiga toxin to induce apoptosis in cancer cells is currently an active area of research.
|Form:||In 1xPBS, PH 7.4. Use sufficient volume to wash down the walls and stopper, if necessary. Handle the product gently, mix by inversion, do not vortex. Read all handling infromation prior to reconsititution.|
|Molecular Mass:||This product migrates as two major bands corresponding to the A and B polypetides, with apparent molecular weights of 32 kDa and 8 kDa, respectively.|
|Storage:||This product is supplied as a lyophilized powder, stoppered under vacuum. Store at 2-8 centigrade.|
|Warning:||Good laboratory technique should be employed in the safe handling of this product. This requlres observing the following practices:
1. Wear appropriate laboratory attire including a lab coat, gloves and safety glasses.
2. Do not mouth pipette, inhale, ingest or allow to come into contact with open wounds. Wash thoroughly any area of the body which comes into contact with the product.
3. Avoid accidental autoinoculation by exercising extreme care when handling in conjunction with any injection device.
4. This product is intended for research purposes by qualified personnel only. It is not intended for use in humans or as a diagnostic agent. Our company is not liable for any damages resulting from the misuse or handling of this product.
Price does not include shipping and packaging costs.Request quote for final price.