Native R. communis Lectin Toxin RCA60, Peroxidase labeled

Cat.No.: Lectin-130R
Product Overview: Native Lectin Toxin RCA60 was isolated from R. communis with peroxidase labeled.
Description: Ricinus communis agglutinin (RCA) occurs in two forms designated RCA60 and RCA120 according to their mol. wt. of approx. 60,000 and 120,000 respectively.1 Neither RCA60 nor RCA120 is blood group specific. RCA60, also referred to as RCAII,2 Ricin D3 or RCL III4 is extremely toxic, inhibits protein synthesis and has an affinity for N-acetyl-D-galactosamine. RCA120, also referred to as RCAI2 or RCL (I + II)4 is an agglutinin and has an affinity for terminal β-D-galactosyl residues.1Conjugates are prepared from affinity purified toxin, RCA60 or agglutinin RCA120.
Source: R. communis
Species: R. communis
Form: Lyophilized powder containing sodium citrate.
Bio-activity: Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 7.2, of a 1 mg protein per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour incubation at 25 centigrade.
Unit Definition: One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20centigrade.
Storage: Store at -20 centigrade
Synonyms: Lectin

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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