"Streptolysin O" Related Products

Native S. pyogenes Streptolysin O

Cat.No.: SLO-175S
Product Overview: Streptolysin O (SLO) is a single polypeptide chain that is not glycosylated. This product is isolated from Streptococcus pyogenes.
Description: Streptolysin O is an immunogenic, oxygen-labile toxin, which is reversibly activated by dithiothreitol. It is released into the extracellular medium along with other toxins, including streptolysin S, during the growth of most strains of group A and many strains of groups C and G Streptococci.
Source: S. pyogenes
Species: S. pyogenes
Form: Lyophilized from a 10 mM Tris-Cl buffer solution containing 3 mM sodium azide, 5 mM sodium EDTA, and 1 mM PMSF.
Unit Definition: One unit will cause 50% lysis of 50 ml of a 2% human red blood cell suspension in phosphate buffered saline, pH 7.4, at 37 centigrade for 30 minutes.
Applications: Permeabilizes membranes to permit cellular uptake of large or charged molecules.
Storage: Store at 2-8 centigrade
Reconstitution: Reconstitute by adding water into the vial and gently rotating. After reconstitution, the solution should be stored at -20 centigrade in aliquots and any unused portions should be discarded. SLO is readily oxidized in solution. The activity is significantly increased in the presence of reducing agents, 20 mM cysteine or 10 mM dithiothreitol. A SLO solution will lose ~50% activity within 10 days when stored at 2-8 centigrade. It is recommended to freeze solutions at a minimal concentration of 0.2 mg/ml.
Synonyms: SLO; Streptolysin O
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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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