"Cholera Toxin" Related Products

Native V. cholerae Cholera Toxin

Cat.No.: CTX-106C
Product Overview: This product is the active, native cholera toxin (composed of the A and the B subunits). It is a lyophilized powder containing ~5% protein (LowryTCA).
Description: Toxin consisting of an A subunit (27 kDa) surrounded by five B subunits (approximately 12 kDa each), which attach the toxin to ganglioside GM1 on the cell surface. The A subunit catalyzes ADP-ribosylation of the α-subunit of the stimulatory G protein (Gαs), reducing GTPase activity and activating the α-subunit. This activation of Gαs leads to an increase in the activity of adenylyl cyclase, resulting in increased levels of cAMP. Also ADP-ribosylates transducin in the eye rod outer segments, inactivating its GTPase activity. Cholera toxin has also been reported to ADP-ribosylate tubulin. Shown to be a potent mucosal vaccine adjuvant, inducing T helper cell type 2 responses by inhibiting the production of interleukin-12.
Source: V. cholerae
Species: V. cholerae
Form: Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide and sodium EDTA.
Purity: ~95% (SDS-PAGE)
Unit Definition: One unit increases adenylate cyclase activity 6-fold, measured as [3H]-cAMP present, using approx. 70,000 CHO cells after incubation for 90 min at 37 centigrade with [3H]-adenine followed by a 15 min incubation with 1 mM 3-isobutyl-1-methylxanthine (IBMX).
Stability: This product is stable 3 years when stored properly.
Storage: Store at 2-8 centigrade
Reconstitution: When reconstituted at 1 mg/mL in water, solution will contain 0.05 M Tris buffer salts, pH 7.5, 0.2 M NaCl, 0.003 M NaN3, and 0.001 M sodium EDTA. Store reconstituted solutions in the refrigerator.
Handling: Cholera toxin is soluble in water at a concentration of 10 mg/ml. Swirl bottles gently during reconstitution. Avoid vigorous pipetting of solutions that may lead to foaming. Solutions can be filtered through a 0.2 mm filter.
Synonyms: CTX; Cholera enterotoxin; Cholergen
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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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