"Cholera Toxin B subunit" Related Products

Native V. cholerae Cholera Toxin B subunit, biotin labeled

Cat.No.: CTB-103C
Product Overview: This product is the cholera toxin B subunit (CTB) labeled with biotin (244.3 Da). The extent of labeling is ~1 mole of biotin per mole of CTB. The conjugate is prepared using (+)-biotin N-hydroxysuccinimide ester. The biotin is conjugated to the lysine residues present in the cholera toxin B subunit. It contains ~2% unconjugated biotin.
Description: Cholera toxin belongs to the AB5-subunit family of toxins. The native hexameric protein has a molecular mass of ~85 kDa and contains two subunits. It consists of a single A subunit (~27.2 kDa), responsible for the ADP-ribosylation activity, and five B subunits (~11.6 kDa each), which are arranged as a pentameric ring with an apparent 5-fold symmetry and are associated with the cell surface receptor binding and subsequent internalization (transmembrane transport) of the enzymatic component.
Source: V. cholerae
Species: V. cholerae
Form: Lyophilized powder containing sodium phosphate buffer salts, sodium azide and sodium EDTA.
Bio-activity: Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding is achieved with 0.02-1 μg of Cholera toxin B subunit-biotin conjugate per mL. The conjugated B subunit gives a similar value for 50% binding to that of unconjugated B subunit from which it is prepared.
Molecular Mass: 12 kDa
Storage: Store at 2-8 centigrade
Handling: Cholera toxin is soluble in water at a concentration of 10 mg/ml. Swirl bottles gently during reconstitution. Avoid vigorous pipetting of solutions that may lead to foaming. Solutions can be filtered through a 0.2 mm filter.
Synonyms: CTB; Cholera Toxin B subunit
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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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