PDE1B Cell-Based Reporter Assay Kit

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PDE1B Cell-Based Reporter Assay Kit

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Cat.No. : Kit-1826
Product Overview : Phosphodiesterases (PDEs) regulate the intracellular levels of cAMP and cGMP by hydrolyzing cAMP and cGMP to their inactive 5 monophosphates. These cyclic nucleotides play an important role as second messengers in diverse physiological functions. PDE1B plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. PDE1B hydrolyzes both cAMP and cGMP to nucleoside 5-prime monophosphate. Although it prefers cGMP at low substrate levels, it has a Vmax that is approximately equal for both cGMP and cAMP (Polli, J.W. and Koncaid, R.L., 1994; Yu, J. et al., 1997).
Size : 500 reactions
Applications : The PDE1B Cell-Based Reporter Assay Kit is designed for screening inhibitors of PDE1B and for monitoring PDE1B/ cGMP/ Ca2+ channel signaling pathway activity in vivo.
Storage : Store cell line in liquid nitrogen immediately upon receipt. Store other components as recommended. Components are stable at least 12 months from date of receipt, when stored as directed.

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