"PPAR" Related Products

        PPARα (mouse) Reporter Assay Kit

        Cat.No. : Kit-2002
        Size : 96 wells
        Description : This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the mouse peroxisome proliferator-activated receptor alpha (NR1C1), a ligand-dependent transcription factor commonly referred to as PPARA or mPPARα. The reporter cells include the luciferase reporter gene functionally linked to a PPARα-responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in mPPARα activity. The principal application of this reporter assay system is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against mPPARα. Mouse PPARα reporter cells are prepared using the proprietary CryoMite process. This cryo-preservation method yields exceptional cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for cumbersome intermediate treatment steps such as spin-and-rinse of cells, viability determinations, cell titer adjustments, or the pre-incubation of reporter cells prior to assay setup. The nuclear receptor reporter assays are all-inclusive cell-based assay systems. In addition to mPPARα reporter cells, this kit provides two optimized media for use during cell culture and in diluting the user"s test samples, a reference agonist, luciferase detection reagent, and a cell culture-ready assay plate.
        Storage : -80°C
        Kit Components : ▪ mPPARα Reporter Cells: 3 x 0.60 mL; -80°C ▪ Cell Recovery Medium (CRM): 1 x 10.5 mL; -20°C▪ Compound Screening Medium (CSM): 1 x 35 mL; -20°C ▪ GW590735, 10 mM (in DMSO) (reference agonist for mPPARα): 1 x 30 µL; -20°C ▪ Detection Substrate: 3 x 2.0 mL; -80°C▪ Detection Buffer: 3 x 2.0 mL; -20°C▪ Plate frame: 1; ambient ▪ Snap-in, 8-well strips (white, sterile, cell-culture ready): 12; ambient

        Online Inquiry

        Note: There will be extra charge for optional service!

        Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

        Optional requirements on this protein    +Expand

        Price Inquiry

        Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.