GRAP

GRB2-related adaptor protein

GRAP; GRB2-related adaptor protein; GRB2-related adapter protein; GRB2 related adaptor protein; Growth factor receptor bound protein 2 related adaptor protein; MGC64880; OTTHUMP00000065766; growth factor receptor-bound protein 2-related adaptor protein;

X-ray Crystallography

1. Introduction X-ray crystallography is a technique used for determining the high-resolution, three-dimensional crystal structures of atom and molecules and has been fundamental in the development of many scientific fields. In its first decades of application, it is mainly used for determining the ...

Protocol of Immobilized Metal Affinity Chromatography

In addition to the enrichment of phosphorylated proteins, phosphorylated peptides can also be enriched. There are many ways to enrich phosphorylated peptides. Immobilized metal affinity chromatography (IMAC) was first proposed by Porath in 1975. After decades of development, it has become the most p...

Guide for Enrichment of Phosphorylated Peptides by Strong Cation Exchange Chromatography

The most commonly used method for the enrichment of phosphorylated peptides is the solid-phase metal ion affinity chromatography (IMAC) described earlier. Although IMAC is relatively simple and fast in application, strong cation exchange chromatography has shown more superior effectiveness in large-...

Guide for Mass Spectrometry Identification of Samples Separated by Liquid Chromatography

As one of the most powerful separation and analysis technologies in the field of chemistry, liquid chromatography (LC) has developed rapidly since the 1970s. Both in terms of fundamental chromatography theory and instrument performance, it has been greatly improved and improved. LC exhibits high sep...