| Description : |
The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of the upstream ZFP91 gene and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. [provided by RefSeq, Oct 2010] |
| Source : |
E. coli |
| Species : |
Mouse |
| Tag : |
His(C-ter) |
| Form : |
Powder |
| Bio-activity : |
Determined by its ability to induce proliferation in TF-1 cells. The ED50 for this effect is < 10 ng/mL. The specific activity of recombinant mouse CNTF is > 1 x 10^5 IU/mg. |
| AA Sequence : |
MAFAEQSPLTLHRRDLCSRSIWLAR KIRSDLTALMESYVKHQGLNKNISL DSVDGVPVASTDRWSEMTEAERLQE NLQAYRTFQGMLTKLLEDQRVHFTP TEGDFHQAIHTLTLQVSAFAYQLEE LMALLEQKVPEKEADGMPVTIGDGG LFEKKLWGLKVLQELSQWTVRSIHD LRVISSHHMGISAHESHYGAKQM |
| Endotoxin : |
Endotoxin level is less than 0.1 EU/μg of the protein, as determined by the LAL test. |
| Purity : |
> 98% (by SDS-PAGE) |
| Applications : |
SDS-PAGE |
| Notes : |
For laboratory research only, not for drug, diagnostic or other use. |
| Storage : |
Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Storage Buffer : |
PBS (pH 7.4) |
| Reconstitution : |
It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
