Recombinant Active Pig IL10 Protein, His-tagged(C-ter)
Cat.No. : | IL10-136P |
Product Overview : | Recombinant Active Pig IL10 Protein with His tag (C-ter) was expressed in E. coli. |
- Specification
- Gene Information
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Description : | The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011] |
Source : | E. coli |
Species : | Pig |
Tag : | His(C-ter) |
Form : | Powder |
Bio-activity : | Determined by its ability to induce proliferation in MC/9‑2 cells. The ED50 for this effect is < 5 ng/mL. |
AA Sequence : | MSIKSENSCIHFPTSLPHMLRELRA AFGPVKSFFQTKDQMGDLLLTGSLL EDFKGYLGCQALSEMIQFYLEDVMP KAESDGEDIKEHVNSLGEKLKTLRL RLRRCHQFLPCENKSKAVEEVKSAF SKLQERGVYKAMGEFDIFINYIEAY MTMKMRKN |
Endotoxin : | Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test. |
Purity : | > 98% (by SDS-PAGE) |
Applications : | SDS-PAGE |
Notes : | For laboratory research only, not for drug, diagnostic or other use. |
Storage : | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
Storage Buffer : | PBS (pH 7.4) |
Reconstitution : | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
Gene Name : | IL10 interleukin 10 [ Sus scrofa ] |
Official Symbol : | IL10 |
Synonyms : | IL10; interleukin 10; interleukin-10; cytokine synthesis inhibitory factor; CSIF; IL-10; |
Gene ID : | 397106 |
mRNA Refseq : | NM_214041 |
Protein Refseq : | NP_999206 |
Products Types
◆ Recombinant Protein | ||
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IL10-999C | Recombinant Chicken IL10 Protein, His&GST-tagged | +Inquiry |
IL10-2678R | Recombinant Rat IL10 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
IL10-2079HCL | Recombinant Human IL10 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionCutting-edge imaging techniques, alongside in vivo disease models, allow for precise assessment of therapeutic effects and tissue responses to IL-10-based treatments.
Advanced technologies, including single-cell RNA sequencing and flow cytometry, enable a granular analysis of IL-10's impact on diverse immune cell subpopulations.
Genotyping studies and genetic association analyses help correlate variations in IL-10 and its receptor with disease susceptibility across diverse populations.
Advanced immunomonitoring tools, including multiplex cytokine assays and immune cell phenotyping, provide comprehensive safety evaluations of IL-10 interventions in clinical trials.
In vitro models involve exposing immune cells to IL-10 and assessing changes in cytokine secretion and cell activation markers to uncover its anti-inflammatory mechanisms.
State-of-the-art techniques such as phosphoproteomics, mass spectrometry, and protein-protein interaction assays provide insights into the intricate signaling cascades activated by IL-10.
Dose-response studies in animal models coupled with outcome measures like cytokine profiles and immune cell phenotypes help determine the optimal IL-10 dosage and administration routes.
Experimental designs encompass co-stimulation experiments, coupled with functional assays and high-dimensional analysis, to elucidate synergistic effects involving IL-10 and other cytokines.
Techniques like RT-PCR, immunohistochemistry, and spatial transcriptomics are employed to characterize IL-10 receptor expression patterns in specific tissues and disease contexts.
CRISPR/Cas9 gene editing is used to create knockout or overexpression models, combined with functional assays, to decipher IL-10's regulatory roles in autoimmune disease pathways.
Customer Reviews (3)
Write a reviewEffective in anti-inflammatory assays.
High purity levels.
Stable under long-term storage.
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