|Product Overview:||Recombinant Anthrax Lethal Factor (LF-A) with an alanine on the N-terminal like the native toxin was expressed in B. anthracis Native Sequence.|
|Description:||Lethal Factor (LF) is the enzymatic subunit of anthrax lethal toxin. When intoxicating a cell, LF binds to PA on the cell surface and translocates through a pore made by seven PA monomers.|
|Form:||When reconstituted with 0.5 ml of sterile purified water contains 1 mg of lethal factor from Bacillus anthracis, native sequence in 5 mM HEPES, 50 mM NaCl, pH 7.5.|
|Molecular Mass:||90 kDa|
|Endotoxin:||The endotoxin content, determined using a kinetic chromagenic LAL assay, is 1.3 EU/mg.|
|Unit Definition:||One unit of lethal factors will catalyze the release of 1.0 umole of cleaved MAPPKKide per minute at 37 centigrade in 20 mM HEPES, pH 8.2.|
|Applications:||Tissue Culture Application: For tissue culture applications, medium containing glutamine must be fresh. Ammonium ion is released when glutamine breaks down, and may prevent acidification of the endosome thereby inhibiting translocation of LF or EF into the cytosol. A stable form of glutamine may be used.|
|Storage:||This product is packaged aseptically, lyophilized and sealed under vacuum. Store at 2-8 centigrade prior to reconstitution.|
|Concentration:||Protein concentration was determined by a modification of the method of Bradford, using bovine serum as the standard.|
|Reconstitution:||Anthrax toxin proteins may be reconstituted in sterile purified water, stored at 2-8 centigrade and used successfully within a few hours. However, over longer periods of time, there may be a decline in the enzymatic activity of LF in vitro and the activity of the protective antigen(PA)+LF complex in living cells. If it is necessary to store this material, reconstitute it at a concentration of 1 mg/ml. Reconstitution with 1 mg/ml BSA will enhance stability and recovery. It is further recommended that the solution is aliquoted and frozen at either -20 centigrade or -80 centigrade. Avoid repeated freeze-thaw cycles. After the protein has been reconstituted as described above, glycerol may be added to 50% if a liquld is desired at freezer temperatures. Storage of material reconstituted with 1 mg/ml BSA at 2-8 centigrade for a period of two weeks may be acceptable for some applications.|
|Warning:||Good laboratory technique should be employed in the safe handling of this product. This requlres observing the following practices:
1. Wear appropriate laboratory attire including a lab coat, gloves and safety glasses.
2. Do not mouth pipette, inhale, ingest or allow to come into contact with open wounds. Wash thoroughly any area of the body which comes into contact with the product.
3. Avoid accidental autoinoculation by exercising extreme care when handling in conjunction with any injection device.
4. This product is intended for research purposes by qualified personnel only. It is not intended for use in humans or as a diagnostic agent. Our company is not liable for any damages resulting from the misuse or handling of this product.
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