"BoNT/C Light Chain" Related Products

Recombinant Botulinum Neurotoxin Type C Light Chain protein

Cat.No.: BoNT/C-157
Product Overview: Recombinant Botulinum Neurotoxin Type C Light Chain(1 - 436 aa) was expressed in E. coli.
Description: The protein was recombinantly expressed in E. coli. The affinity tag has been cleaved from the protein prior to quantitation and packaging.
Source: E. coli
Species: C. botulinum
Form: Each vial of Light Chain from Botulin um Neurotoxin Type C (BoNT/C) contains 10 ug of lyophilized protein. When reconstituted with 100 ul of water, each vial contains 10 ug of lig ht chain in 20 mM HEPES, pH 7.4, 1.25% lactose. In order to ensure stability during storage and recovery of the protein, 0.05% TWEEN-20 or 1 mg/ml BSA must be included in the reconstitution buffer.
Bio-activity: The BoNTIC light chain has been tested for activity in a FRET based assay. Digestion of the FRET substrate was detected within two hours when using ten and five nanomolar BoNTIC Light Chain with 10uM SYNTAXtide substrate.
Molecular Mass: ESI-MS analysis shows the molecular weight to be 50.874 kDa.
Protein length: 436 amino acids
Purity: >95%
Notes: Toxicity: The light chain of Botulinum Neurotoxin is non-toxic and unable to penetrate cells in the absence of the heavy chain. The expression and purification of light chain from a recombinant setting ensures there is no possible contamination with heavy chain or full length intact toxin.
Storage: This product is supplied as a lyophilized powder which has been stoppered under vacuum. Store lyophilized vials at 2-8 centigrade. Once dissolved, aliquot and store the product at -20 centigrade. Refrain from multiple freezelthaw cycles.
Warning: Good laboratory technique should be employed in the safe handling of this product. Wear appropriate laboratory attire including a lab coat, gloves and safety glasses. Nitrile gloves are recommended when handling lyophilized material. This product is intended for research purposes by qualified personnel only. It is not intended for use in humans or as a diagnostic agent.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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