Active Recombinant Canine IL1B

• Cat.No.: IL1B-656C
• Product Overview: Recombinant Canine IL1B (Accession # NP_001033060) Ala114-Ser265 was produced in E. coli-derived.

Specification

• Source: E. coli
• Species: Canine
• Predicted N Terminal: Ala114
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272.The ED50 for this effect is typically 4 - 16 pg/mL.
• Molecular Mass: Recombinant Canine IL1B has a calculated MW of 18 kDa.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 10 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: IL1B interleukin 1, beta [ Canis lupus familiaris (dog) ]
• Official Symbol: IL1B
• Synonyms: IL1B; interleukin 1 beta; IL-1; interleukin-1 beta; IL-1 beta; interleukin-1 beta proprotein
• Gene ID: 403974
• mRNA Refseq: NM_001037971
• Protein Refseq: NP_001033060
• UniProt ID: Q28292
• Chromosome Location: 17