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Active Recombinant COVID-19 (BA.2.3.20) Spike RBD protein, His-tagged

Cat.No. : Spike-804V
Product Overview : Recombinant COVID-19 (BA.2.3.20) Spike RBD protein(YP_009724390.1, with mutation G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, K444R, N450D, L452M, N460K, S477N, T478K, E484R, Q498R, N501Y, Y505H) (Arg319-Phe541) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.
  • Specification
  • Gene Information
  • Related Products
Source : HEK293
Species : COVID-19
Tag : His
Form : Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : Immobilized human ACE2 protein (Fc tag) at 2 μg/mL (100 μL/well) can bind Recombinant Recombinant SARS-CoV-2 (BA.2.3.20) Spike RBD Protein (His Tag), the EC50 is 6-18 ng/mL.
Molecular Mass : The recombinant SARS-CoV-2 Spike RBD consists of 234 amino acids and predicts a molecular mass of 26.83 kDa. As a result of glycosylation, it migrates as an approximately 35.54 kDa band in SDS-PAGE under reducing conditions.
Protein Length : Arg319-Phe541
Endotoxin : < 1.0 EU per μg protein as determined by the LAL method.
Purity : > 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended to reconstitute the lyophilized powder in sterile water to 100-200 ug/ml, and further dilute with other solvents. The dissolved protein can be stored at 4°C for 2-7 days and should be used up as soon as possible. If not in use for a short time, please store at -20°C.
Gene Name : S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
Official Symbol : S
Synonyms : Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; COVID-19 Omicron
Gene ID : 43740568
Protein Refseq : YP_009724390.1
UniProt ID : P0DTC2

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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