Active Recombinant COVID-19 Plpro protein

• Cat.No.: Plpro-11V
• Product Overview: Recombinant 2019-nCoV Papain-like Protease is produced by our E. coli expression system and the target gene encoding Glu1564-Lys1878 is expressed.

Specification

• Description: Replication of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) requires proteolytic processing of the replicase polyprotein by two viral cysteine proteases, a chymotrypsin-like protease (3CLpro) and a papain-like protease (PLpro). These proteases are important targets for development of antiviral drugs that would inhibit viral replication and reduce mortality associated with outbreaks of SARSCoV. PLpro is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region. In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity. In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons.
• Source: E. coli
• Species: COVID-19
• Tag: N/A
• Form: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 10 mM 2-Mercaptoethanol, 20% Glycerol, pH 7.5.
• Bio-activity: Measured by its ability to cleave a fluorogenic peptide substrate, (EEDANS)RELNGGAPI(K-DABCYL)S (Synthetic). The specific activity is >2000pmol/min/mg.
• Molecular Mass: 34kDa, reducing conditions.
• Protein length: Glu1564-Lys1878
• Purity: Greater than 90% as determined by reducing SDS-PAGE.
• Storage: Store at <-20°C, stable for 6 months after receipt.
  Please minimize freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μl. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Shipping: The product is shipped on dry ice/polar packs.
  Upon receipt, store it immediately at the temperature listed below.

Gene Information

• Gene Name: ORF1ab
• Official Symbol: ORF1ab
• Synonyms: Papain-like Protease; PLpro; PL-PRO; pp1a; Replicase polyprotein 1a
• Gene ID: 43740578
• Protein Refseq: YP_009724389.1
• UniProt ID: P0DTC1