Active Recombinant Cynomolgus CYP3A5 Protein

• Cat.No.: CYP3A5-129C
• Product Overview: Recombinant cynomolgus CYP3A5 protein and human CYP-reductase without tag were coexpressed in E. coli.

Specification

• Description: The contribution of the CYP3A5 enzyme to the metabolism of clinically used drugs has been established only for a few CYP3A substrates, such as the immunosuppressant tacrolimus, while for drugs used in the field of psychiatry its role is still vague.
• Source: E. coli
• Species: Cynomolgus
• Bio-activity: Cytochrome c Reductase Activity: 347 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: Testosterone 6β-hydroxylase
  Linear with time up to: 30 min(Determined at 40 µM substrate, using a P450 concentration of 12.5 pmol/ml)
  Linear with P450 up to: 60 pmol/ml(Determined at 40 µM substrate, using an incubation time of 5 min)
  Vmax: 5.8 pmol/min/pmol CYP
  Km: 16 μM
• Usage: For laboratory (research) purposes only
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 8.6 nmol/ml
  Protein Concentration: 9.8 mg/ml
  Specific P450 Content: 878 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: CYP3A5 cytochrome P450 family 3 subfamily A member 5 [ Macaca fascicularis (crab-eating macaque) ]
• Official Symbol: CYP3A5
• Synonyms: CYP3A5; cytochrome P450 family 3 subfamily A member 5; cytochrome P450 3A5; cytochrome P450 family 3 subfamily A member 5; cytochrome P450, family 3, subfamily A, polypeptide 5
• Gene ID: 102146134
• mRNA Refseq: NM_001319511
• Protein Refseq: NP_001306440
• UniProt ID: A8CBR0