| Description : |
BMP-2 protein, like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. BMP-2 protein is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. BMP-2 and BMP-7 are osteogenic BMPs that have been demonstrated to potently induce osteoblast differentiation in a variety of cell types. BMP-2, BMP-4 and BMP-7 are known to be of major importance in bone formation and repair. In cancerous tissues BMP-2 protein may play an important role in the progression of glioma. |
| Source : |
E. coli |
| Species : |
Danio rerio (zebrafish) |
| Predicted N Terminal : |
Met |
| Form : |
Lyophilized from sterile 30mM HAC, pH 3.0. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Bio-activity : |
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is typically 0.5-3 μg/mL. |
| Molecular Mass : |
The recombinant zebrafish BMP2A consists of 116 amino acids and has a calculated molecular mass of 13.2 kDa. It migrates as an 13 KDa band in SDS-PAGE under reducing conditions. |
| Protein length : |
Gln272-Arg386 |
| Endotoxin : |
< 1.0 EU per μg protein as determined by the LAL method. |
| Purity : |
> 95 % as determined by SDS-PAGE |
| Storage : |
Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
| Official Symbol : |
BMP2A |
| Synonyms : |
BMP2A;bone morphogenetic protein 2 |
