Recombinant DENV (type 4, strain Philippines/H241/1956) Envelope protein, His-tagged

• Cat.No.: Envelope-353D
• Product Overview: Recombinant DENV Envelope protein (type 1, Met280-Gly674), fused to His-tag at C-terminus, was expressed in Baculovirus-Insect Cells.

Specification

• Description: The protein plays a role in virus budding by binding to the cell membrane and gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle. During virus entry, may induce genome penetration into the host cytoplasm after hemifusion induced by the surface proteins. Can migrate to the cell nucleus where it modulates host functions. Overcomes the anti-viral effects of host EXOC1 by sequestering and degrading the latter through the proteasome degradation pathway.
• Source: Baculovirus-Insect Cells
• Species: DENV
• Tag: His
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10% gly, pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant envelope protein of Dengue virus (DENV)(type 4, strain Philippines/H241/1956) consists of 406 amino acids and predicts a molecular mass of 44.8 kDa.
• Protein length: 406
• Endotoxin: <1.0 EU per μg protein as determined by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20 centigrade to -80 centigrade
  Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2mg/ml. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: POLY
• Official Symbol: POLY
• Synonyms: polyprotein gene; E Protein; DENV
• Gene ID: 5075729
• Protein Refseq: NP_073286.1
• UniProt ID: P09866