Active Recombinant Equine IL5 Protein, His-tagged

• Cat.No.: IL5-901E
• Product Overview: Recombinant Equine IL5 (Accession # O02699) Leu20-Gly134, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Equine
• Tag: His
• Predicted N Terminal: Leu20
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.The ED50 for this effect is typically 4 - 16 ng/mL.
• Molecular Mass: Recombinant Equine IL5, His-tagged has a calculated MW of 13.9 kDa (monomer). In SDS-PAGE migrates as 19 kDa, reducing conditions.
• Protein length: Leu20-Gly134
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: IL5 interleukin 5 (colony-stimulating factor, eosinophil) [ Equus caballus (horse) ]
• Official Symbol: IL5
• Synonyms: IL-5; interleukin-5; interleukin-5; T-cell replacing factor; TRF; eosinophil differentiation factor
• Gene ID: 1.00034e+008
• mRNA Refseq: NM_001082499.1
• Protein Refseq: NP_001075968.1
• UniProt ID: O02699
• Chromosome Location: chromosome: 14