Active Recombinant Equine TNF, Met-tagged

• Cat.No.: TNF-666E
• Product Overview: Recombinant Equine TNF (Accession # NP_001075288) Leu78-Leu234, fused with an N-terminal Met, was produced in E. coli-derived.

Specification

• Source: E. coli
• Species: Equine
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221.The ED50 for this effect is typically 0.025-0.1 ng/mL.
• Molecular Mass: Recombinant Equine TNF, Met-tagged has a calculated MW of 17.2 kDa.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 25 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: TNF tumor necrosis factor [ Equus caballus (horse) ]
• Official Symbol: TNF
• Synonyms: TNF; tumor necrosis factor; TNFA; TNF-a; TNFSF2; tumor necrosis factor; TNF-alpha; cachectin; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
• Gene ID: 100033834
• mRNA Refseq: NM_001081819
• Protein Refseq: NP_001075288
• UniProt ID: P29553
• Chromosome Location: 20