Active Recombinant Feline IL1B protein(Ala116-Ser267)

• Cat.No.: IL1B-1030F
• Product Overview: Recombinant Feline IL1B (NP_001070882.1) (Ala116-Ser267) was expressed in E. coli with an initial Met.

Specification

• Source: E. coli
• Species: Feline
• Tag: N/A
• Protein length: Ala116-Ser267
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 2-8 ng/mL.
• Molecular Mass: The recombinant Feline IL1B consists 153 amino acids and predicts a molecular mass of 17.5 kDa.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.