Active Recombinant Full Length Human MAPK10 Protein, GST-tagged

• Cat.No.: MAPK10-8439HF
• Product Overview: Full-length human JNK3 [1-426(end) amino acids of accession number NP_620446.1] was expressed as N-terminal GST-fusion protein (75 kDa) using E.coli expression system. GST-JNK3 was purified by using glutathione sepharose chromatography and activated with His-tagged MAP2K4 and MAP2K7. Activated GST-JNK3 was purified using glutathione sepharose chromatography.

Specification

• Source: E. coli
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
• Protein length: 1-426
• Purity: 86%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: mitogen-activated protein kinase 10
• Official Symbol: MAPK10
• Synonyms: MAPK10;JNK3;JNK3A;PRKM10;SAPK1b;p493F12;p54bSAPK
• Protein Refseq: NP_620446.1
• UniProt ID: P53779