"HA" Related Products

Recombinant H10N3 (A/mallard/Minnesota/Sg-00194/2007) HA Protein, His-tagged

Cat.No.: HA-706V
Product Overview: Recombinant Influenza A virus (A/mallard/Minnesota/Sg-00194/2007(H10N3)) hemagglutinin (ACT84107.1) (Met1-Val525) was expressed with a C-terminal polyhistidine tag.
Source: Baculovirus-Insect cells
Species: H10N3
Tag: His
Predicted N Terminal: Leu 17
Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: 1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System. 2. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 2-8 ng/mL for 1% GRBC.
Molecular Mass: The recombinant hemagglutinin of Influenza A virus (A/mallard/Minnesota/Sg-00194/2007(H10N3)) comprises 520 amino acids and has a predicted molecular mass of 57.9 kDa. The apparent molecular mass of the protein is approximately 64 kDa in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >90 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.

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