Recombinant H1N1 (A/Puerto Rico/8/34/Mount Sinai) NS1 Protein, His-tagged

Cat.No.: NS1-805V
Product Overview: Recombinant influenza A H1N1 Virus (A/Puerto Rico/8/34/Mount Sinai) NS1 protein (C8XP22) (Asp 2-Val 230) was expressed, with a polyhistidine tag at the N-terminus.
Source: E. coli
Species: H1N1
Tag: His
Predicted N Terminal: Met
Form: Lyophilized from sterile PBS, 5% glycerol, pH 8.0, 5% ~ 8% trehalose and mannitol.
Molecular Mass: The recombinant influenza A H1N1 Virus (A/Puerto Rico/8/34/Mount Sinai) NS1 protein comprises 240 amino acids and has a predicted molecular mass of 27.2 kDa. It migrates as an approximately 29 kDa band in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >95 % as determined by SDS-PAGE
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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