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Recombinant H2N2 (A/Guiyang/1/1957) HA Protein, His-tagged

Cat.No.: HA-644V
Product Overview: Recombinant Influenza A virus (A/Guiyang/1/1957(H2N2)) hemagglutinin (ACD85231.1) (Met1-Arg340), termed as HA1, was expressed with a C-terminal polyhistidine tag.
Source: HEK293
Species: H2N2
Tag: His
Predicted N Terminal: Asp 16
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Molecular Mass: The recombinant HA1 subunit of Influenza A virus (A/Guiyang/1/1957(H2N2)) comprises 336 amino acids and has a predicted molecular mass of 37.8 kDa. The apparent molecular mass of the protein is approximately 49.2 kDa in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >95 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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