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Recombinant H5N1 (A/Egypt/2321-NAMRU3/2007) HA Protein, His-tagged

Cat.No.: HA-359V
Product Overview: Recombinant extracellular domain (Met 1-Gln 531) of the influenza A H5N1 Hemagglutinin (A/Egypt/2321-NAMRU3/2007) (ABP96850.1) (HA1+HA2, uncleaved, with the cleavage site mutated (RRRKR-TETR) was expressed, fused with a C-terminal polyhistidine tag.
Source: HEK293
Species: H5N1
Tag: His
Predicted N Terminal: Asp 17
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Molecular Mass: The secreted recombinant influenza A H5N1 HA (A/Egypt/2321-NAMRU3/2007 (H5N1)) comprises 524 amino acids and has a predicted molecular mass of 60 kDa. As a result of glycosylation, it migrates as an approximately 65-80 kDa band in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >97 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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