"HA" Related Products


Recombinant H7N7 (A/chicken/Netherlands/1/03) HA Protein, His-tagged

Cat.No.: HA-305V
Product Overview: Recombinant extracellular domain of Influenza A virus (A/chicken/Netherlands/1/03 (H7N7)) (AAR02639.1) hemagglutinin (Met 8-Phe 544) (Native, HA1+HA2) was expressed, fused with a polyhistidine tag at the C-terminus.
Source: Baculovirus-Insect Cells
Species: H7N7
Tag: His
Predicted N Terminal: Asp 26
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: 1. Measured in hemagglutination test using 1% chicken erythrocyte. The HA titer is 0.1-0.5 μg/ml for 1% CRBC.2. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System.
Molecular Mass: The secreted recombinant hemagglutinin of Influenza A virus (A/chicken/Netherlands/1/03 (H7N7)) comprises 530 amino acids and has a calculated molecular mass of 59.4 kDa as estimated in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >80 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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