Recombinant HIV gp160 (subtype CRF01_AB, strain 07JP_NMC716_clone_01) Protein, His & MBP-tagged

• Cat.No.: gp160-636V
• Product Overview: Recombinant HIV-2 envelope glycoprotein gp160 (BAM76170.1)(Gln547-Tyr670), termed as gp36, was expressed with the N-terminal polyhistidine-tagged MBP tag at the N-terminus.

Specification

• Source: E. coli
• Species: HIV
• Tag: His & MBP
• Predicted N Terminal: Met
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Molecular Mass: The recombinant HIV-2 gp36 consists of 524 amino acids and has a predicted molecular mass of 58.8 kDa. The apparent molecular mass of the recombinant protein is approximately 55 KDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.