Active Recombinant Human ACHE Protein, His-tagged
Cat.No. : | ACHE-04H |
Product Overview : | Recombinant human ACHE (32-614aa), fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques. |
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Description : | Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. AChE activity may constitute a sensitive biomarker of RBC ageing in vivo, and thus, may be of aid in understanding the effects of transfusion. |
Source : | HEK293 |
Species : | Human |
Tag : | His |
Form : | Liquid |
Bio-activity : | Specific activity is > 6,000 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1.0 nmol acetylthiocholine per minute at pH7.5 at 25 centigrade. |
Molecular Mass : | 65.6 kDa (592aa) |
Protein length : | 32-614aa |
AA Sequence : | |
Endotoxin : | < 1 EU/μg of protein (determined by LAL method) |
Purity : | > 95% by SDS-PAGE |
Applications : | SDS-PAGE, Enzyme Activity |
Storage : | Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles. |
Concentration : | 0.25 mg/mL (determined by Absorbance at 280nm) |
Storage Buffer : | Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol |
Gene Name : | ACHE acetylcholinesterase (Cartwright blood group) [ Homo sapiens (human) ] |
Official Symbol : | ACHE |
Synonyms : | ACHE; acetylcholinesterase (Cartwright blood group); YT; ACEE; ARACHE; N-ACHE; acetylcholinesterase; Yt blood group; acetylcholinesterase (Yt blood group); apoptosis-related acetylcholinesterase; EC 3.1.1.7 |
Gene ID : | 43 |
mRNA Refseq : | NM_000665 |
Protein Refseq : | NP_000656.1 |
MIM : | 100740 |
UniProt ID : | P22303 |
Products Types
◆ Recombinant Protein | ||
ACHE-36R | Recombinant Rhesus Macaque ACHE Protein, His (Fc)-Avi-tagged | +Inquiry |
Ache-511M | Recombinant Mouse Ache Protein, MYC/DDK-tagged | +Inquiry |
ACHE-106R | Recombinant Rat ACHE Protein, His (Fc)-Avi-tagged | +Inquiry |
ACHE-2415H | Recombinant Human ACHE Protein, His (Fc)-Avi-tagged | +Inquiry |
ACHE-6969C | Recombinant Chicken ACHE | +Inquiry |
◆ Native Protein | ||
ACHE-8345H | Native Human ACHE | +Inquiry |
◆ Lysates | ||
ACHE-2163MCL | Recombinant Mouse ACHE cell lysate | +Inquiry |
◆ Assay kits | ||
Kit-0033 | Acetylcholinesterase Green Fluorescence Assay Kit | +Inquiry |
Kit-0032 | Acetylcholinesterase Assay Kit | +Inquiry |
Kit-0031 | Acetylcholinesterase Activity Colorimetric Assay Kit | +Inquiry |
Kit-0034 | Acetylcholinesterase Red Fluorescence Assay Kit | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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