Active Recombinant Human ADAMTS5 Protein, His-tagged
Cat.No. : | ADAMTS5-144H |
- Specification
- Gene Information
- Related Products
Description : | Synthetic human ADAMTS5 DNA sequence, amino acid residues 626-930 with N-terminal signal peptide and C-terminal 6His-Tag expressed in the CHO cell expression system. This protein binds to murine monoclonal antibodies of defined epitope specificity. ADAMST5: ADAMST5 defined as a disintegrin-metalloproteinase with thrombospondin motif 5, also known as aggrecanase-2 and ADAMST511, is a member of the family of secreted zinc proteases with a multi-domain structure. The protein precursor consists of signal peptide , a pro-catalytic, disintegrin-like, TS type 1 motif, cysteine-rich, spacer and a variable number of TS type 1 motifs. ADAMST5 is an active protease effectively cleaving alpha-2 macroglobulin, aggrecan and brevican, and is inhibited by TIMP-3. The purified rhADAMSt5 starts at the N-terminus of the catalytic domain and ends at the C-terminus of the TSP-1 domain. The amino acid sequence of rhADAMST5 has 95-98% homology with canine, bovine and murine proteins. |
Source : | CHO cell |
Species : | Human |
Tag : | His |
Form : | Frozen Liquid |
Bio-activity : | Measured by its ability to cleave human Aggrecan (native and recombinant). Substrate and enzyme in a molar ratio of 10:1 (10ug subtrate, 1ug enzyme) in buffer (50mM Tris, pH 7.5, 10mM CaCl2, 0.15M NaCl, 0.05% Brij-35 incubated for 2-4 hours at 37C. The degradation products were analyzed by SDS-PAGE Western blots using epitope-specific monoclonal antibodies and molecular mass determination of aggrecan degradation products. |
Molecular Mass : | 54 kD |
Purity : | > 95 % by SDS-PAGE |
Applications : | Aggrecanase Assays; Drug Screening. |
Notes : | Recommended concentrations for use are approximate values. A substrate/enzyme assays sould be performed to determine the optimal ratios for specific applications. |
Stability : | This protein is stable for upto 12 months at -75 centigrade. Aliquote protein for long term use and freeze at -75 centigrade. Avoid repeated freezing –thawing of the protein. Endotoxin content of the protein: <1000EU/mg. |
Storage : | Store at -75 centigrade. |
Dilutions : | 20mM Na-Ac, 0.6M NaCl, 0.01M CaCll2, 0.035% Brij-35 |
Products Types
◆ Recombinant Protein | ||
ADAMTS5-2425H | Recombinant Human ADAMTS5 Protein, His (Fc)-Avi-tagged | +Inquiry |
ADAMTS5-2037M | Recombinant Mouse ADAMTS5 Protein (262-930 aa), His-tagged | +Inquiry |
ADAMTS5-0159H | Recombinant Human ADAMTS5 Protein (Glu485-Pro622), N-His-tagged | +Inquiry |
ADAMTS5-534H | Active Recombinant Human ADAMTS5, His-tagged | +Inquiry |
ADAMTS5-306H | Active Recombinant Human ADAMTS5 Protein, His-tagged | +Inquiry |
◆ Lysates | ||
ADAMTS5-9028HCL | Recombinant Human ADAMTS5 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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