| Description : |
This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
85.1 kDa |
| AA Sequence : |
MASVHESLYFNPMMTNGVVHANVFG IKDWVTPYKIAVLVLLNEMSRTGEG AVSLMERRRLNQLLLPLLQGPDITL SKLYKLIEESCPQLANSVQIRIKLM AEGELKDMEQFFDDLSDSFSGTEPE VHKTSVVGLFLRHMILAYSKLSFSQ VFKLYTALQQYFQNGEKKTVEDADM ELTSRDEGERKMEKEELDVSVREEE VSCSGPLSQKQAEFFLSQQASLLKN DETKALTPASLQKELNNLLKFNPDF AEAHYLSYLNNLRVQDVFSSTHSLL HYFDRLILTGAESKSNGEEGYGRSL RYAALNLAALHCRFGHYQQAELALQ EAIRIAQESNDHVCLQHCLSWLYVL GQKRSDSYVLLEHSVKKAVHFGLPY LASLGIQSLVQQRAFAGKTANKLMD ALKDSDLLHWKHSLSELIDISIAQK TAIWRLYGRSTMALQQAQMLLSMNS LEAVNAGVQQNNTESFAVALCHLAE LHAEQGCFAAASEVLKHLKERFPPN SQHAQLWMLCDQKIQFDRAMNDGKY HLADSLVTGITALNSIEGVYRKAVV LQAQNQMSEAHKLLQKLLVHCQKLK NTEMVISVLLSVAELYWRSSSPTIA LPMLLQALALSKEYRLQYLASETVL NLAFAQLILGIPEQALSLLHMAIEP ILADGAILDKGRAMFLVAKCQVASA ASYDQPKKAEALEAAIENLNEAKNY FAKVDCKERIRDVVYFQARLYHTLG KTQERNRCAMLFRQLHQELPSHGVP LINHLTRTRPLEQKLISEEDLAAND ILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
