| Description : |
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
57.4 kDa |
| AA Sequence : |
MLRVVSWNINGIRRPLQGVANQEPS NCAAVAVGRILDELDADIVCLQETK VTRDALTEPLAIVEGYNSYFSFSRN RSGYSGVATFCKDNATPVAAEEGLS GLFATQNGDVGCYGNMDEFTQEELR ALDSEGRALLTQHKIRTWEGKEKTL TLINVYCPHADPGRPERLVFKMRFY RLLQIRAEALLAAGSHVIILGDLNT AHRPIDHWDAVNLECFEEDPGRKWM DSLLSNLGCQSASHVGPFIDSYRCF QPKQEGAFTCWSAVTGARHLNYGSR LDYVLGDRTLVIDTFQASFLLPEVM GSDHCPVGAVLSVSSVPAKQCPPLC TRFLPEFAGTQLKILRFLVPLEQSP VLEQSTLQHNNQTRVQTCQNKAQVR STRPQPSQVGSSRGQKNLKSYFQPS PSCPQASPDIELPSLPLMSALMTPK TPEEKAVAKVVKGQAKTSEAKDEKE LRTSFWKSVLAGPLRTPLCGGHREP CVMRTVKKPGPNLGRRFYMCARPRG PPTDPSSRCNFFLWSRPSTRTRPLE QKLISEEDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
