Recombinant Human ARHGAP25 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ARHGAP25-945H
• Product Overview: ARHGAP25 MS Standard C13 and N15-labeled recombinant protein (NP_055697) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases, which are implicated in actin remodeling, cell polarity, and cell migration.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 72.3 kDa
• AA Sequence: MSLGQSACLFLSIARSRSVMTGEQM AAFHPSSTPNPLERPIKMGWLKKQR SIVKNWQQRYFVLRAQQLYYYKDEE DTKPQGCMYLPGCTIKEIATNPEEA GKFVFEIIPASWDQNRMGQDSYVLM ASSQAEMEEWVKFLRRVAGTPCGAV FGQRLDETVAYEQKFGPHLVPILVE KCAEFILEHGRNEEGIFRLPGQDNL VKQLRDAFDAGERPSFDRDTDVHTV ASLLKLYLRDLPEPVVPWSQYEGFL LCGQLTNADEAKAQQELMKQLSILP RDNYSLLSYICRFLHEIQLNCAVNK MSVDNLATVIGVNLIRSKVEDPAVI MRGTPQIQRVMTMMIRDHEVLFPKS KDIPLSPPAQKNDPKKAPVARSSVG WDATEDLRISRTDSFSSMTSDSDTT SPTGQQPSDAFPEDSSKVPREKPGD WKMQSRKRTQTLPNRKCFLTSAFQG ANSSKMEIFKNEFWSPSSEAKAGEG HRRTMSQDLRQLSDSQRTSTYDNVP SLPGSPGEEASALSSQACDSKGDTL ASPNSETGPGKKNSGEEEIDSLQRT VQELRKEIETQKQMYEEQIKNLEKE NYDVWAKVVRLNEELEKEKKKSAAL EISLRNMERSREDVEKRNKALEEEV KEFVKSMKEPKTEATRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ARHGAP25 Rho GTPase activating protein 25 [ Homo sapiens (human) ]
• Official Symbol: ARHGAP25
• Synonyms: ARHGAP25; Rho GTPase activating protein 25; rho GTPase-activating protein 25; KIAA0053; rho-type GTPase-activating protein 25; KAIA0053
• Gene ID: 9938
• mRNA Refseq: NM_014882
• Protein Refseq: NP_055697
• MIM: 610587
• UniProt ID: P42331