| Cat. No. : |
BIRC2-3104H |
| Description : |
Baculoviral IAP repeat-containing protein 2, also known as Inhibitor of apoptosis protein 2, HIAP2, c-IAP1, RING finger protein 48, and BIRC2, is a member of the IAP family. The inhibitor of apoptosis (IAP) proteins are a family of anti-apoptotic regulators found in viruses and metazoans. The UBA (ubiquitin-associated) domain of IAPs is located between the BIR (baculovirus IAP repeat) domains and the CARD (caspase activation and recruitment domain) or the RING (really interesting new gene) domain of c-IAP1 and c-IAP2 or XIAP (X-linked IAP) respectively. c-IAP1 contains three BIR repeats, one CARD domain and one RING-type zinc finger. c-IAP1 and c-IAP2 are recruited to tumor necrosis factor receptor 1 (TNFR1) associated complexes where they can regulate receptor-mediated signaling. Both c-IAP1 and c-IAP2 have been implicated in TNFalpha-stimulated NF-kappaB activation. Treatment of cells with IAP antagonists leads to proteasomal degradation of c-IAP1 and c-IAP2. Deletion or mutation of the UBA domain decreases this degradation, probably by diminishing the interaction of the c-IAPs with the proteasome. Ubiquitin binding may be an important mechanism for rapid turnover of auto-ubiquitinated c-IAP1 and c-IAP2. |
| Formulation : |
Lyophilized from sterile 10mM Tris, 5% glycerol, 0.5mM EDTA, 5mM DTT, pH 7.5 |
| Purity : |
> 92 % as determined by SDS-PAGE |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method. |
| Bio-activity : |
Measured by its ability to inhibit DEVD-AFC cleavage activity in cell extracts activated by addition of cytochrome c and dATP. |
| Stability : |
Samples are stable for up to twelve months from date of receipt at -70°C. |
| Predicted N terminal : |
Gly |
| Molecular Mass : |
The recombinant human cIAP1 ( Glu 144-Leu 356 ) consists of 230 amino acids and has a calculated molecular mass of 26.5 kDa as estimated by SDS-PAGE under reducing conditions. |
| Storage : |
Store it under sterile conditions at -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
