Recombinant Human CAPN2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: CAPN2-3550H
• Product Overview: CAPN2 MS Standard C13 and N15-labeled recombinant protein (NP_001739) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 80 kDa
• AA Sequence: MAGIAAKLAKDREAAEGLGSHERAI KYLNQDYEALRNECLEAGTLFQDPS FPAIPSALGFKELGPYSSKTRGIEW KRPTEICADPQFIIGGATRTDICQG ALGDCWLLAAIASLTLNEEILARVV PLNQSFQENYAGIFHFQFWQYGEWV EVVVDDRLPTKDGELLFVHSAEGSE FWSALLEKAYAKINGCYEALSGGAT TEGFEDFTGGIAEWYELKKPPPNLF KIIQKALQKGSLLGCSIDITSAADS EAITFQKLVKGHAYSVTGAEEVESN GSLQKLIRIRNPWGEVEWTGRWNDN CPSWNTIDPEERERLTRRHEDGEFW MSFSDFLRHYSRLEICNLTPDTLTS DTYKKWKLTKMDGNWRRGSTAGGCR NYPNTFWMNPQYLIKLEEEDEDEED GESGCTFLVGLIQKHRRRQRKMGED MHTIGFGIYEVPEELSGQTNIHLSK NFFLTNRARERSDTFINLREVLNRF KLPPGEYILVPSTFEPNKDGDFCIR VFSEKKADYQAVDDEIEANLEEFDI SEDDIDDGFRRLFAQLAGEDAEISA FELQTILRRVLAKRQDIKSDGFSIE TCKIMVDMLDSDGSGKLGLKEFYIL WTKIQKYQKIYREIDVDRSGTMNSY EMRKALEEAGFKMPCQLHQVIVARF ADDQLIIDFDNFVRCLVRLETLFKI FKQLDPENTGTIELDLISWLCFSVL SGPTRTRPLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: CAPN2 calpain 2 [ Homo sapiens (human) ]
• Official Symbol: CAPN2
• Synonyms: CAPN2; calpain 2, (m/II) large subunit; calpain-2 catalytic subunit; CANPL2; CANPml; mCANP; CANP 2; M-calpain; calpain M-type; millimolar-calpain; calpain-2 large subunit; calpain 2, large subunit; calpain large polypeptide L2; calpain, large polypeptide L2; calpain 2, large [catalytic] subunit; calcium-activated neutral proteinase 2; CANP2; FLJ39928
• Gene ID: 824
• mRNA Refseq: NM_001748
• Protein Refseq: NP_001739
• MIM: 114230
• UniProt ID: P17655