Recombinant Human CCT4 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: CCT4-6363H
• Product Overview: CCT4 MS Standard C13 and N15-labeled recombinant protein (NP_006421) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The chaperonin containing TCP1 complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin and tubulin, as well as alpha-transducin.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 57.7 kDa
• AA Sequence: MPENVAPRSGATAGAAGGRGKGAYQ DRDKPAQIRFSNISAAKAVADAIRT SLGPKGMDKMIQDGKGDVTITNDGA TILKQMQVLHPAARMLVELSKAQDI EAGDGTTSVVIIAGSLLDSCTKLLQ KGIHPTIISESFQKALEKGIEILTD MSRPVELSDRETLLNSATTSLNSKV VSQYSSLLSPMSVNAVMKVIDPATA TSVDLRDIKIVKKLGGTIDDCELVE GLVLTQKVSNSGITRVEKAKIGLIQ FCLSAPKTDMDNQIVVSDYAQMDRV LREERAYILNLVKQIKKTGCNVLLI QKSILRDALSDLALHFLNKMKIMVI KDIEREDIEFICKTIGTKPVAHIDQ FTADMLGSAELAEEVNLNGSGKLLK ITGCASPGKTVTIVVRGSNKLVIEE AERSIHDALCVIRCLVKKRALIAGG GAPEIELALRLTEYSRTLSGMESYC VRAFADAMEVIPSTLAENAGLNPIS TVTELRNRHAQGEKTAGINVRKGGI SNILEELVVQPLLVSVSALTLATET VRSILKIDDVVNTRTRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: CCT4 chaperonin containing TCP1 subunit 4 [ Homo sapiens (human) ]
• Official Symbol: CCT4
• Synonyms: CCT4; chaperonin containing TCP1, subunit 4 (delta); T-complex protein 1 subunit delta; Cctd; TCP-1-delta; stimulator of TAR RNA-binding; chaperonin containing t-complex polypeptide 1, delta subunit; SRB; CCT-DELTA; MGC126164; MGC126165
• Gene ID: 10575
• mRNA Refseq: NM_006430
• Protein Refseq: NP_006421
• MIM: 605142
• UniProt ID: P50991