Active Recombinant Human CD209 protein

• Cat.No.: CD209-635H
• Product Overview: Recombinant Human CD209(Gln59-Ala404) fused with YPYDVPDYA at N-terminal was expressed in CHO.

Specification

• Description: Human Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209 is a member of the C-type lectin family. The canonical DC-SIGN/CD209 isoform is a 46 kDa, 404 amino acid (aa) type II transmembrane protein. The extracellular region contains a Ca2+-dependent carbohydrate-binding lectin domain. Multiple human DC-SIGN/CD209 splice forms exist, generating both membrane-bound and soluble forms. DC-SIGN/CD209 is not well conserved between mouse and human, with the extracellular domain sharing only 63% aa identity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs. DC-SIGN/CD209 binds to butyrophilin 2A1 and this interaction can be blocked by HIV pp120. DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation.
• Source: CHO
• Species: Human
• Predicted N Terminal: Tyr
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. The ED50 for this effect is typically 0.4-2 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 41 kDa;
  SDS-PAGE: 41-48 kDa, reducing conditions.
• Protein length: Gln59-Ala404
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 250 μg/mL in PBS.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: CD209 CD209 molecule [ Homo sapiens ]
• Official Symbol: CD209
• Synonyms: CD209; CD209 molecule; CD209 antigen; CDSIGN; CLEC4L; DC SIGN; DC SIGN1; HIV gpl20-binding protein; C-type lectin domain family 4 member L; C-type lectin domain family 4, member L; dendritic cell-specific ICAM-3-grabbing non-integrin 1; dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin; DC-SIGN; DC-SIGN1; MGC129965
• Gene ID: 30835
• mRNA Refseq: NM_001144893
• Protein Refseq: NP_001138365
• MIM: 604672
• UniProt ID: Q9NNX6
• Chromosome Location: 19p13
• Pathway: Measles, organism-specific biosystem; Measles, conserved biosystem; Phagosome, organism-specific biosystem; Phagosome, conserved biosystem; Tuberculosis, organism-specific biosystem; Tuberculosis, conserved biosystem
• Function: mannose binding; metal ion binding; peptide antigen binding; receptor activity; sugar binding; virion binding