Active Recombinant Human CD274 protein, mFc-tagged
Cat.No. : | CD274-1003H |
Product Overview : | Recombinant Human CD274 protein(Phe 19 - Arg 238), fused with mFc tag, was expressed in HEK293. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Human |
Tag : | mFc |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. |
Bio-activity : | Immobilized Human PD-1, Strep Tag at 1 μg/mL (100 μL/well) can bind Human PD-L1 Protein, Mouse IgG1 Fc Tag with a linear range of 5-78 ng/mL.Serial dilutions of Nivolumab were added into Human PD-L1 Protein, Mouse IgG1 Fc Tag: Human PD-1, Strep Tag binding reactions. The half maximal inhibitory concentration (IC50) is 0.2391 μg/mL. |
Molecular Mass : | This protein carries a mouse IgG1 Fc tag at the C-terminusThe protein has a calculated MW of 51.4 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Protein Length : | Phe 19 - Arg 238 |
Endotoxin : | Less than 0.1 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS. |
Storage : | For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | CD274 CD274 molecule [ Homo sapiens ] |
Official Symbol : | CD274 |
Synonyms : | CD274; CD274 molecule; CD274 antigen , PDCD1LG1, programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296; |
Gene ID : | 29126 |
mRNA Refseq : | NM_014143 |
Protein Refseq : | NP_054862 |
MIM : | 605402 |
UniProt ID : | Q9NZQ7 |
Products Types
◆ Recombinant Protein | ||
CD274-558R | Recombinant Rhesus Macaque CD274 Protein, His (Fc)-Avi-tagged | +Inquiry |
CD274-051H | Active Recombinant Human CD274 protein(Met1-Thr239), His&Avi-tagged, Biotinylated | +Inquiry |
CD274-0763H | Recombinant Human CD274 Protein, His-Flag-StrepII-Tagged | +Inquiry |
CD274-74H | Recombinant Human CD274 Protein, Biotin Labeled | +Inquiry |
CD274-709H | Recombinant Human CD274 Protein, Fc-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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