|Product Overview:||Recombinant Human CD274 Protein, MIgG1 Fc-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1). Predicted N-terminus: Phe 19. This protein carries a mouse IgG1 Fc tag at the C-terminus , and has a calculated MW of 51.6 kDa. The predicted N-terminus is Phe 19. The reducing (R) protein migrates as 65-85 kDa in SDS-PAGE due to glycosylation and the non-reducing (NR) protein migrates as 130-170 kDa.|
|Tag :||MIgG1 Fc|
|Form:||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.|
|Bio-activity:||Immobilized Recombinant Human CD274 Protein, mIgG1 Fc-tagged, low endotoxin at 5 μg/mL ( 100 μl/well ) can bind Biotinylated Human PD-1, His Tag & Fc tag with a linear range of 0.05-0.2 μg/mL (Routinely tested).
Immobilized Human PD-1, Tag Free, Full Length at 0.2 μg/mL ( 100 μl/well ) can bind Recombinant Human CD274 Protein, mIgG1 Fc-tagged, low endotoxin with a linear range of 2-80 ng/mL (QC tested).
Flow Cytometry assay shows that Recombinant Human CD274 Protein, mIgG1 Fc-tagged, low endotoxin can bind to 293 cell overexpressing human PD-1. The concentration of PD-L1 used is 0.1ug/ml (Routinely tested).
FACS analysis shows that the binding of Recombinant Human CD274 Protein, mIgG1 Fc-tagged, low endotoxin to 293 overexpressing PD-1 was inhibited by increasing concentration of neutralizing anti-PD-1 antibody. The concentration of PD-L1 used is 1ug/ml. The IC50 is 0.145 μg/mL (Routinely tested).
|Molecular Mass:||51.6 kDa|
|Protein length:||Phe 19 - Arg 238|
|Endotoxin:||Less than 0.1 EU per μg by the LAL method.|
|Purity:||>92% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
|Notes:||Please avoid repeated freeze-thaw cycles.|
|Stability:||No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Storage:||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.|
|Reconstitution:||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.|
|Gene Name:||CD274 CD274 molecule [ Homo sapiens ]|
|Synonyms:||CD274; CD274 molecule; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296;|