Recombinant Human CD274 Protein, MIgG1 Fc-tagged, low endotoxin

Cat.No.: CD274-364H
Product Overview: Recombinant Human CD274 Protein, MIgG1 Fc-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1). Predicted N-terminus: Phe 19. This protein carries a mouse IgG1 Fc tag at the C-terminus , and has a calculated MW of 51.6 kDa. The predicted N-terminus is Phe 19. The reducing (R) protein migrates as 65-85 kDa in SDS-PAGE due to glycosylation and the non-reducing (NR) protein migrates as 130-170 kDa.
Source: HEK293
Species: Human
Tag : MIgG1 Fc
Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity: Immobilized Recombinant Human CD274 Protein, mIgG1 Fc-tagged, low endotoxin at 5 μg/mL ( 100 μl/well ) can bind Biotinylated Human PD-1, His Tag & Fc tag with a linear range of 0.05-0.2 μg/mL (Routinely tested).
Immobilized Human PD-1, Tag Free, Full Length at 0.2 μg/mL ( 100 μl/well ) can bind Recombinant Human CD274 Protein, mIgG1 Fc-tagged, low endotoxin with a linear range of 2-80 ng/mL (QC tested).
Flow Cytometry assay shows that Recombinant Human CD274 Protein, mIgG1 Fc-tagged, low endotoxin can bind to 293 cell overexpressing human PD-1. The concentration of PD-L1 used is 0.1ug/ml (Routinely tested).
FACS analysis shows that the binding of Recombinant Human CD274 Protein, mIgG1 Fc-tagged, low endotoxin to 293 overexpressing PD-1 was inhibited by increasing concentration of neutralizing anti-PD-1 antibody. The concentration of PD-L1 used is 1ug/ml. The IC50 is 0.145 μg/mL (Routinely tested).
Molecular Mass: 51.6 kDa
Protein length: Phe 19 - Arg 238
Endotoxin: Less than 0.1 EU per μg by the LAL method.
Purity: >92% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Notes: Please avoid repeated freeze-thaw cycles.
Stability: No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Gene Name: CD274 CD274 molecule [ Homo sapiens ]
Official Symbol: CD274
Synonyms: CD274; CD274 molecule; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296;
Gene ID: 29126
mRNA Refseq: NM_014143
Protein Refseq: NP_054862
MIM: 605402
UniProt ID: Q9NZQ7

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.