| Description : |
Human B7 homolog 3 (B7-H3), also known as CD276, is a member of the B7 family of immune checkpoint molecules, responsible for regulating immune responses (1‑3). There are at least seven structurally related B7 family members, all sharing about 20-40% amino acid (aa) sequence identity (4). The B7 protein family are immunoglobulin (Ig) superfamily members with varying numbers of Ig-V-like and Ig-C-like regions in the extracellular domain (ECD) and they can either be glycosylphosphatidylinositol (GPI)-linked or transmembrane (4). The mature ECD of B7-H3 contains two V-like and two C-like Ig domains, a transmembrane region, and a short cytoplasmic domain. An isoform of human B7-H3 containing only a single set of V-like and C-like Ig domains in the ECD has also been identified (1, 5). The ECD of both mouse and rat B7-H3 only contain a single set of V-like and C-like Ig domains. Human B7-H3 is not expressed on resting B cells, T cells, monocytes or dendritic cells, but is induced on dendritic cells and monocytes by inflammatory cytokines (1, 6). B7-H3 is also overexpressed in numerous cancers including bladder, breast and melanoma (7). Unlike other B7 family members, human B7-H3 does not bind any known members of the CD28 family of immunoreceptors and its receptor has yet to be identified. However, B7-H3 has been shown to bind an unidentified counter-receptor on activated T cells to co-stimulate the proliferation of CD4+ or CD8+ T cells (8). B7-H3 has also been found to enhance the induction of primary cytotoxic T lymphocytes and stimulate IFN-gamma production (1-3, 8). |
| Source : |
CHO cells |
| Species : |
Human |
| Tag : |
Fc/Avi |
| Predicted N Terminal : |
Leu29 |
| Form : |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Bio-activity : |
The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect is 0.1-1 μg/mL. Measured by its binding ability in a functional ELISA. When Human CD276 Antibody is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant CD276 Fc Chimera Avi-tag that produces 50% of the optimal binding response is approximately 10-60 ng/mL. |
| Molecular Mass : |
104-120 kDa, under reducing conditions |
| Protein length : |
Leu29-Thr461 |
| Endotoxin : |
<0.10 EU per 1 μg of the protein by the LAL method. |
| Purity : |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| Applications : |
Binding Activity, Bioactivity |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Reconstitution : |
Reconstitute at 200 μg/mL in PBS. |
