Active Recombinant Human CD33 Protein, Fc-tagged, Alexa Fluor® 647 conjugated

• Cat.No.: CD33-26H
• Product Overview: Recombinant human Alexa Fluor® 647 conjugated Siglec-3 (Asp18-His259)(Val257Leu) protein with human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in mouse myeloma cell line.

Specification

• Description: Enables protein phosphatase binding activity and sialic acid binding activity. Involved in several processes, including negative regulation of cytokine production; negative regulation of monocyte activation; and positive regulation of protein tyrosine phosphatase activity. Located in several cellular components, including Golgi apparatus; external side of plasma membrane; and peroxisome.
• Source: Mouse myeloma cell line
• Species: Human
• Tag: Fc
• Form: Disulfide-linked homodimer
  Labeled with Alexa Fluor® 647 via amines
  Excitation Wavelength: 650 nm
  Emission Wavelength: 668 nm
• Bio-activity: Measured by flow cytometry for its ability to bind anti-human Siglec-3/CD33 Monoclonal Antibody conjugated beads. The concentration of Recombinant Human Siglec-3/CD33 Fc Chimera Alexa Fluor® 647 that produces 50% of the binding response is 0.50-20.0 ng/mL.
• Molecular Mass: 53.4 kDa
• N-terminal Sequence Analysis: Asp18
• Endotoxin: <1.0 EU/μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after opening.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Shipping: The product is shipped with dry ice or equivalent.

Gene Information

• Gene Name: CD33 CD33 molecule [ Homo sapiens (human) ]
• Official Symbol: CD33
• Synonyms: CD33; CD33 molecule; p67; SIGLEC3; SIGLEC-3; myeloid cell surface antigen CD33; CD33 antigen (gp67); CD33 molecule transcript; gp67; sialic acid-binding Ig-like lectin 3
• Gene ID: 945
• mRNA Refseq: M23197
• Protein Refseq: AAA51948
• MIM: 159590
• UniProt ID: P20138